Conserving species in their natural habitat for their own intrinsic value and to maintain their role in ecosystem and evolutionary development is the principle of in-situ conservation. Nepal has established a network of twenty protected areas for conserving viable populations of the large and wide-ranging species in their wilderness. In situ conservation in Nepal is focused on umbrella and keystone species: Bengal tigers, Greater one-horned rhinoceros, Asian elephants and Snow leopards have come a long way since the early years of conservation in Nepal. Since then, NTNC has continually worked together with the government as the main supporting agency to conduct species research and provide technical assistance in species conservation and protected area management. NTNC has been a key partner in the government-led initiatives in conducting national tiger surveys, monitoring of recovering species such as rhinos, their translocation, and managing species focused recovery sites to revive locally endangered species such as the Blackbuck. With its dedicated technical staff at the field level, NTNC continues to adopt cutting edge technology and use contemporary science to strengthen in-situ conservation. NTNC's focus are also beyond protected areas where special efforts to minimize community disservice from wildlife and enhancing local livelihood to gain support for conservation finds its ultimate outcome in conservation at the species level.